Details, Fiction and Phos-tag Acrylamide AAL-107
Details, Fiction and Phos-tag Acrylamide AAL-107
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2nd gel electrophoresis: Phosphorylated sorts with identical isoelectric factors or molecular weights may be divided.
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Phos-tag Acrylamide AAL-107 is employed for the separation of phosphorylated proteins utilizing a self-designed acrylamide gel. The acrylamide-pendant Phos-tag ligand may be used for mobility change detection of phosphorylated proteins using phosphate affinity SDS-Web page. The method is based on a traditional SDS-Webpage but Phos-tag is incorporated into the gel which retards the development with the phosphorylated proteins and therefore enables the separation of phosphorylated and non-phosphorylated molecules.
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